FastQCFastQC Report
Thu 2 Jun 2016
DRR024875_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024875_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4525333
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100420.22190632159003548No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85830.1896656002994697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50420.11141721504251731No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67100.052.1951371
GTATCAA127900.041.2399981
ATCAACG155000.033.5110863
TCAACGC155700.033.3600584
CAACGCA163400.031.8732555
TATCAAC164950.031.7478832
AACGCAG171750.030.485186
ACGCAGA191250.027.1799857
CGCAGAG193850.026.5966388
GTGGTAT33000.025.499721
GTACATG131850.025.2078381
GCAGAGT209450.024.0098449
TACATGG131200.023.5762082
TGGTATC34050.023.3319742
TACCTGG36550.023.1508542
ACATGGG134850.022.0653423
GTACCTG40450.020.9194491
AGAGTAC196500.020.53353110-11
GAGTACT140700.020.32523512-13
CAGAGTA207500.019.97728210-11
Produced by FastQC (version 0.11.5)