FastQCFastQC Report
Thu 2 Jun 2016
DRR024875_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024875_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4525333
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115520.25527403176738594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100070.22113289784420287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65700.14518268600343887No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73300.053.9049571
GTATCAA135500.040.9286961
ATCAACG167900.032.7050673
TCAACGC169250.032.3537184
CAACGCA175100.031.245955
TATCAAC176950.031.1120952
AACGCAG184600.029.6862536
ACGCAGA208250.025.9978127
CGCAGAG210700.025.5170738
GTGGTAT32550.024.4224431
GTACATG125150.023.528641
GCAGAGT226150.023.3789639
TACATGG125500.022.2143632
TGGTATC34400.022.0034282
ACATGGG128300.021.216553
AGAGTAC209650.020.53371410-11
CAGAGTA221050.020.32517410-11
GAGTACT159200.020.17715612-13
TACCTGG34500.018.9416852
AGTACTT167650.018.79575312-13
Produced by FastQC (version 0.11.5)