FastQCFastQC Report
Thu 2 Jun 2016
DRR024874_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024874_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503048
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91460.2610869163083121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77370.22086480116744045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46440.13257026452392318No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55650.057.3600541
GTATCAA104800.040.686561
ATCAACG128500.032.996753
TCAACGC130850.032.367764
CAACGCA136050.031.0956295
TATCAAC136200.031.0644872
ATAACGC3050.030.8246333
AACGCAG141450.030.0052156
GTGGTAT25300.029.5448381
TGGTATC25800.027.5141452
ACGCAGA158250.026.6111647
CGCAGAG159350.026.1915058
GTACATG82950.025.0501791
TACCTGG27050.023.983392
TACATGG83000.023.788662
GCAGAGT174350.023.506839
TATAACG3400.023.5054632
ACATGGG84500.022.1964743
GAGTACT126900.021.23976312-13
AGAGTAC159700.021.02794810-11
Produced by FastQC (version 0.11.5)