FastQCFastQC Report
Thu 2 Jun 2016
DRR024874_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024874_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503048
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102940.2938583770476454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91820.2621145927774898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57390.16382875712807818No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57350.057.830911
GTATCAA106400.039.350721
ATCAACG133850.030.8781643
TCAACGC134950.030.5789664
GTGGTAT25950.029.7310871
CAACGCA139850.029.6083765
TATCAAC140050.029.5464522
TGGTATC25150.029.5409812
AACGCAG146200.028.5136366
GTACCGG3500.025.5381761
ACGCAGA164800.025.0666127
CGCAGAG165550.024.8678868
TACCTGG28500.023.758742
GTACATG85400.023.1914841
GCAGAGT180850.022.3482699
TACATGG85650.022.0701082
GTACCTG32500.021.5678671
GTCTCGC10400.021.2602731
TCGCTCT10850.021.2259674
AGAGTAC172350.020.38280110-11
Produced by FastQC (version 0.11.5)