FastQCFastQC Report
Thu 2 Jun 2016
DRR024873_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024873_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194695
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84950.2659095782226472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72640.22737694834718183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42500.13303304384299597No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53300.056.2701761
GTATCAA98300.040.3142741
ATCAACG115150.033.921973
TCAACGC116750.033.457094
TATCAAC121700.032.2517662
CAACGCA122750.031.9360925
AACGCAG128450.030.6633726
GTGGTAT23050.028.3484081
ATAACGC3500.028.2029973
ACGCAGA142600.027.25777
CGCAGAG142850.027.1441928
TATAACG3350.026.6603852
TACAACG6750.026.4628982
TACCTGG25400.026.2792052
GTCTCGC9050.025.4526081
TGGTATC24200.025.0571672
GTACATG72200.024.8069041
GTACCTG28400.024.4978621
GCAGAGT154800.024.4718789
TACATGG73200.022.9895022
Produced by FastQC (version 0.11.5)