FastQCFastQC Report
Thu 2 Jun 2016
DRR024873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194695
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97960.30663334058493846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85620.26800680503146623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55150.17262993806920535No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54900.056.0234451
GTATCAA101400.040.1183051
ACAACGC5450.034.504163
ATCAACG128700.031.5604523
TCAACGC130650.031.0086964
TATAACG2800.030.2228982
TATCAAC133750.030.1237222
CAACGCA135550.030.0957975
TGGTATC23950.029.052122
GTGGTAT25300.028.998031
AACGCAG141500.028.9290226
ATAACGC3000.028.2071553
TACCTGG26500.027.853222
TACAACG7700.025.643672
CGCAGAG158650.025.4751538
ACGCAGA160050.025.3408037
GTACATG74950.024.4713211
GTACCTG30150.024.333341
TACATGG75850.022.9333632
GCAGAGT173000.022.9273999
Produced by FastQC (version 0.11.5)