FastQCFastQC Report
Thu 2 Jun 2016
DRR024872_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024872_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4201559
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99900.23776888531138085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82740.19692690260924578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52630.1252630273667465No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79350.046.0307041
GTATCAA142850.038.8307041
ATCAACG172200.031.7740573
TCAACGC173900.031.4090064
TATCAAC182500.030.1356812
CAACGCA181750.030.1296445
AACGCAG188850.029.070876
ACGCAGA210350.025.7404587
CGCAGAG210300.025.6568788
GTACATG125650.024.953811
GCAGAGT224800.023.7092559
TACATGG123400.023.6175352
ACATGGG126850.022.1226273
GTGGTAT44400.021.5983581
AGAGTAC201050.021.12152510-11
GAGTACT143400.020.5981812-13
AGTACTT151650.019.43112212-13
CAGAGTA224300.019.39314810-11
TGGTATC44550.019.3090722
GTACCTG33800.018.4973051
Produced by FastQC (version 0.11.5)