FastQCFastQC Report
Thu 2 Jun 2016
DRR024872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4201559
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115740.27546917703642865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96420.2294862454626961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66650.158631593653689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90050.045.2334181
GTATCAA154500.037.537021
ATCAACG193050.029.5628663
TCAACGC194700.029.2574254
TATCAAC201100.028.59022
CAACGCA201700.028.405165
AACGCAG210200.027.3226266
ACGCAGA232500.024.417557
CGCAGAG232450.024.2408458
GCAGAGT247750.022.2316689
GTACATG127300.022.132151
AGAGTAC219600.021.48619510-11
ACAACGC7050.021.3382363
TACATGG123900.021.323962
GAGTACT159800.020.4834212-13
TATAACG4150.020.390472
TAACGCA4200.020.1429334
AGTACTT167950.020.11902812-13
ACATGGG128000.019.9428653
CAGAGTA246850.018.71451210-11
Produced by FastQC (version 0.11.5)