FastQCFastQC Report
Thu 2 Jun 2016
DRR024871_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024871_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4311493
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109280.25346208378396995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90970.21099419620999038No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53000.12292725512948763No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70750.053.7505571
GTATCAA127350.039.8644451
ATCAACG156150.031.699133
TCAACGC157650.031.3967914
CAACGCA166200.029.8661125
TATCAAC168700.029.6474592
AACGCAG173850.028.7922346
GTACATG142400.026.9364971
TACATGG137300.025.9170912
ACGCAGA193500.025.5280257
CGCAGAG194450.025.1854768
ACATGGG139450.024.8096283
TGGTATC31300.024.6297742
GTGGTAT32100.024.6017061
GCAGAGT209350.022.966419
CATGGGG79550.020.4449844
AGAGTAC195100.020.37996910-11
CAGAGTA203950.020.19847510-11
GAGTACT142350.019.99112-13
GAGTACA102000.019.9087751
Produced by FastQC (version 0.11.5)