FastQCFastQC Report
Thu 2 Jun 2016
DRR024871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4311493
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122860.28495929368318584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107640.2496582970214726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68660.15924877994699285No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74100.054.6496541
GTATCAA132750.040.425331
ATCAACG164700.032.256783
GTGGTAT31750.031.9972381
TCAACGC166950.031.7287374
TATCAAC173450.030.9013042
CAACGCA175050.030.3948215
AACGCAG184400.028.9794166
TGGTATC33000.028.9220032
GTACATG139800.026.5780051
TACATGG136800.025.3982962
ACGCAGA209900.025.2340227
CGCAGAG210150.025.0698188
ACATGGG140150.023.817793
GCAGAGT226850.022.706329
TATAACG4050.020.8960272
GAGTACT157600.020.51668412-13
CATGGGG80500.019.9101164
CAGAGTA223450.019.85484910-11
AGAGTAC214000.019.73237210-11
Produced by FastQC (version 0.11.5)