FastQCFastQC Report
Thu 2 Jun 2016
DRR024870_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024870_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3112183
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75560.24278777951039512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65220.20956351217136013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38860.12486412270743719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46150.051.3383331
GTATCAA86200.041.0648881
ATCAACG104900.033.2483023
TCAACGC105500.033.1483234
TACCTGG26850.032.388922
TATCAAC110500.031.7355142
CAACGCA111000.031.6747115
AACGCAG114750.030.8838776
GTGGTAT21900.027.9050081
GTACCTG34050.026.7835141
ACGCAGA132150.026.5320537
GTACATG102850.025.73281
TGGTATC23100.025.4370672
CGCAGAG137000.025.2497128
ACCTGGG34500.025.0691623
TACAACG6350.023.688922
TACATGG104750.023.6496962
ACATGGG104650.022.8622843
TAACGCA2905.456968E-1222.6919144
GCAGAGT148100.022.4686729
Produced by FastQC (version 0.11.5)