FastQCFastQC Report
Thu 2 Jun 2016
DRR024870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3112183
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90010.2892182111398976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77340.24850723752427153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52510.16872401140935478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50300.052.922291
GTATCAA91750.042.8539051
TATAACG3300.039.8883252
ATCAACG113950.034.1599923
TCAACGC114500.033.9472054
CAACGCA118800.032.837175
TATCAAC119600.032.6641652
TACCTGG28650.032.6535342
AACGCAG122150.031.9740476
TAACGCA3800.030.9214924
ATAACGC4550.027.896783
GTGGTAT20600.027.8536951
GTACCTG33500.027.797811
ACGCAGA143250.026.869867
CGCAGAG148600.025.8392188
ACCTGGG34850.025.6302323
GTACATG99900.025.092961
TACATGG102400.023.5056212
GCAGAGT163150.023.0739299
TGGTATC23750.022.3674532
Produced by FastQC (version 0.11.5)