FastQCFastQC Report
Thu 2 Jun 2016
DRR024869_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024869_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5022204
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102400.20389454510410168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85620.17048291945130065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51030.10160877574865537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78200.050.799091
GTATCAA145150.042.2020341
ATCAACG174650.034.559883
TCAACGC176150.034.291934
CAACGCA185100.032.608445
TATCAAC187000.032.3032262
AACGCAG193300.031.2953036
ACGCAGA217300.027.341077
CGCAGAG217400.027.0249988
GTACATG140950.024.7816221
GCAGAGT236300.024.3860789
TATAACG4200.023.5046832
TACATGG138650.023.4283962
TGGTATC39150.022.5741042
ACATGGG142150.021.7928643
GTGGTAT42100.021.7750191
GAGTACT156450.020.95304112-13
AGAGTAC217900.020.22223910-11
CAGAGTA235050.019.9165610-11
ATAACGC5700.018.9683133
Produced by FastQC (version 0.11.5)