FastQCFastQC Report
Thu 2 Jun 2016
DRR024869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5022204
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113230.2254587826380609No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100740.20058922337682816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62300.12404912265610873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77600.050.8667031
GTATCAA141300.041.087181
ATCAACG178850.032.3112643
TCAACGC181000.031.837794
TATCAAC187400.030.7878232
CAACGCA188750.030.555445
AACGCAG197650.029.3204946
ACGCAGA226150.025.4571597
CGCAGAG226850.025.25388
GTACATG136250.023.6875951
GCAGAGT249500.022.4526469
TACATGG137550.021.7762362
ACAACGC8950.021.5403043
ACATGGG135750.021.1637383
AGAGTAC227050.020.99869710-11
GTGGTAT40100.020.297151
GAGTACT168700.019.91832212-13
TGGTATC39400.019.4533942
AGTACTT179900.019.30523512-13
CAGAGTA245850.018.74300410-11
Produced by FastQC (version 0.11.5)