FastQCFastQC Report
Thu 2 Jun 2016
DRR024868_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024868_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643779
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87280.2395315412927074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76260.20928821424131377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43100.11828379273276451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56400.056.348931
GTATCAA113950.043.856811
ATCAACG138400.035.6299933
TCAACGC138950.035.4541554
CAACGCA144400.034.2462465
TATCAAC145250.034.1781042
AACGCAG150700.032.9678346
ACGCAGA169550.029.0796077
CGCAGAG169450.028.7639148
TGGTATC25950.028.4421542
GTGGTAT28750.026.6543351
GCAGAGT184650.025.581599
TATAACG3050.023.1202032
GTACATG100350.021.2226141
CAGAGTA180300.021.08936910-11
AGAGTAC170300.020.97537410-11
GAGTACT127200.020.82033712-13
TACATGG100400.019.7128122
ACATGGG101300.019.2581713
AGTACTT133700.018.87660412-13
Produced by FastQC (version 0.11.5)