FastQCFastQC Report
Thu 2 Jun 2016
DRR024868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643779
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100420.27559300385671026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89890.24669443454172166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56450.154921580040941No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62100.056.8877371
GTATCAA114200.040.1202051
TGGTATC27550.031.7433622
ATCAACG146700.030.9592723
TCAACGC147600.030.7922044
GTGGTAT29500.030.13771
TATCAAC152300.029.7603972
CAACGCA154550.029.4379125
AACGCAG161900.028.2462436
ACGCAGA181400.024.9234777
CGCAGAG181950.024.6931618
GTACCGG3100.022.7614481
GTACATG97600.022.749011
GCAGAGT196600.022.4467229
TACATGG99350.021.5803552
AGAGTAC185400.020.3552610-11
GAGTACT139450.019.91772812-13
ACATGGG102600.019.796123
CAGAGTA195050.019.75780710-11
TACCTGG28400.019.5355322
Produced by FastQC (version 0.11.5)