FastQCFastQC Report
Thu 2 Jun 2016
DRR024867_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024867_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3271779
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92140.28162048842541015No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73050.22327302669281757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46320.14157435450255046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69600.044.103821
TATAACG4000.043.481792
GTATCAA114650.035.5891761
ATAACGC5050.033.5101853
TACCTGG31900.032.8609282
TAACGCA5800.032.4188544
GTACCTG33450.031.6197681
ATCAACG135450.029.8806343
TCAACGC136950.029.6220024
TATCAAC141800.028.5093862
CAACGCA142150.028.505335
AACGCAG148300.027.7001956
GTCTCGC9800.027.3414211
TCTGTCG12950.026.8576418
GTACATG83450.025.6305181
GTATAAC9800.024.943051
ACGCAGA163050.024.876887
CGCAGAG162000.024.6029268
ACAACGC6900.024.5255663
TACAACG7000.024.1752012
Produced by FastQC (version 0.11.5)