FastQCFastQC Report
Thu 2 Jun 2016
DRR024867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3271779
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101150.30915902327143735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86350.26392369411259137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60100.18369211367882732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC6300.038.061893
TATAACG3600.037.8758932
GGTATCA78800.035.6370281
GTATCAA125550.033.6069451
GTACCTG34550.033.35581
TACCTGG33750.033.017332
TACAACG8000.031.1496542
ATAACGC4250.029.8700223
TAACGCA4500.027.1557774
ATCAACG154250.026.9760763
TCAACGC155450.026.7882524
TATCAAC159900.026.140912
CAACGCA162150.025.7683285
AACGCAG169300.024.873636
ACCTGGG46350.023.9399343
GTACATG84450.023.8395161
GTACAAC12350.023.6144621
GTCTCGC10850.022.9773241
TCTGTCG11900.022.5110688
TACATGG84850.022.387052
Produced by FastQC (version 0.11.5)