FastQCFastQC Report
Thu 2 Jun 2016
DRR024866_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024866_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4922529
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137070.27845442860773395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115810.23526524678676347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70570.14336126816114236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86750.054.630571
GTATCAA161650.043.5402341
ATCAACG204750.034.0737043
TCAACGC206700.033.683684
TATCAAC210450.033.353232
CAACGCA219050.031.7624955
AACGCAG229050.030.3910666
GTGGTAT37800.027.1149881
ACGCAGA255800.026.8816627
CGCAGAG257900.026.4432688
GTACATG195000.024.617041
GCAGAGT274800.024.064489
TGGTATC40700.023.6802622
TACATGG196900.023.518932
ACATGGG203050.022.1342223
GAGTACT163950.020.8686712-13
AGAGTAC253000.020.56488210-11
CAGAGTA265650.020.48791910-11
GTACTTT174100.019.58414514-15
AGTACTT169200.019.58229412-13
Produced by FastQC (version 0.11.5)