FastQCFastQC Report
Thu 2 Jun 2016
DRR024866_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024866_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4922529
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162870.3308665119088176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138590.2815422722750846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91660.18620509904563284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98850.053.0172351
GTATCAA179450.042.784381
ATCAACG232150.032.8114433
TCAACGC233450.032.5963334
TATCAAC238500.031.9979672
CAACGCA243350.031.4054435
AACGCAG255100.030.0676146
GTGGTAT42200.028.4273471
ACGCAGA291950.025.9491887
CGCAGAG292250.025.761748
GTACATG205250.024.7083741
TGGTATC46500.024.6734072
GCAGAGT311750.023.6528639
TACATGG208150.023.629122
ACATGGG211950.022.2508453
TACCTGG39650.021.4648382
AGAGTAC289150.020.81236510-11
GAGTACT192050.020.21289412-13
CAGAGTA302900.019.99947710-11
ACAACGC9600.019.5915053
Produced by FastQC (version 0.11.5)