FastQCFastQC Report
Thu 2 Jun 2016
DRR024865_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024865_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4201772
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153840.3661312417713289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126200.3003494716038852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75670.1800906855488589No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88650.056.5302541
GTATCAA163850.042.4923861
ATCAACG207200.033.3255773
TCAACGC210150.032.9021074
TATCAAC213650.032.4538152
CAACGCA221600.031.2020685
AACGCAG231950.029.9295886
GTGGTAT37950.028.2439581
TGGTATC38050.027.0561562
GTACATG192700.026.4941861
ACGCAGA258850.026.473647
CGCAGAG256900.026.3995298
TACATGG191050.025.8602262
ACATGGG197700.023.9662173
GCAGAGT277900.023.8464959
TACCTGG36850.022.9621052
GAGTACT170300.021.097512-13
GTACCTG42850.020.7354531
CAGAGTA272900.020.6408910-11
CATGGGA98600.020.546644
Produced by FastQC (version 0.11.5)