FastQCFastQC Report
Thu 2 Jun 2016
DRR024865_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024865_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4201772
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169510.40342503115352285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143950.3425935533865236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94390.22464331715285837No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91500.058.440581
GTATCAA170850.041.2080351
GTGGTAT36750.033.400911
ATCAACG221700.031.3209423
TGGTATC38350.030.892652
TATCAAC226700.030.837522
TCAACGC225150.030.7699384
CAACGCA235050.029.4939465
AACGCAG245150.028.450016
GTACATG192800.026.6861171
TACATGG193250.025.3980852
ACGCAGA281100.024.559867
CGCAGAG281100.024.425828
ACATGGG196350.023.943583
TACCTGG35600.022.8463122
GCAGAGT296500.022.5865529
CATGGGG89650.020.8657444
GAGTACT190400.020.4249412-13
GTACCTG42700.020.045581
AGTACTT194500.020.00647212-13
Produced by FastQC (version 0.11.5)