FastQCFastQC Report
Thu 2 Jun 2016
DRR024864_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024864_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3210232
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111340.34682851582066343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92300.28751816068122177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55930.17422416822210982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66950.052.8730551
GTATCAA119250.041.2741621
TACCTGG31600.033.4702032
ATCAACG146350.033.339113
TCAACGC147650.033.1092454
TATCAAC151850.032.194482
CAACGCA155450.031.5991155
GTGGTAT29500.031.5524561
AACGCAG161950.030.4150896
GTACCTG37900.029.3967361
TGGTATC31050.028.6129822
ACGCAGA182450.026.7400537
CGCAGAG185300.026.1250448
GTACATG128900.026.039661
ACCTGGG39400.025.2923413
TACATGG130050.025.0487352
GCAGAGT192900.024.2917189
ACATGGG131350.023.86963
TACAACG7200.022.8506452
TAGACCG1954.6741843E-721.6948815
Produced by FastQC (version 0.11.5)