FastQCFastQC Report
Thu 2 Jun 2016
DRR024864_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024864_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3210232
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126880.3952362321477077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108240.33717189287254007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71710.22337949406771848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68000.055.128421
GTATCAA122050.040.0023731
TACCTGG30800.037.858022
GTACCTG37250.032.5776371
ACAACGC6700.030.87653
ATCAACG156150.030.8023473
TCAACGC157850.030.46164
TATCAAC160550.030.1929322
ACCTGGG38300.029.7075443
CAACGCA164600.029.3837475
AACGCAG172150.028.1479136
GTGGTAT28250.027.6385841
GTACATG129050.025.2945881
TGGTATC30400.025.209892
TATAACG3600.024.8146442
ACGCAGA197650.024.3012337
TACATGG128300.024.2965052
CGCAGAG200750.023.8549968
TACAACG8250.023.3661332
ACATGGG129700.022.9463653
Produced by FastQC (version 0.11.5)