FastQCFastQC Report
Thu 2 Jun 2016
DRR024863_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024863_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4187012
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103030.24607046743596625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90780.21681332654408442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51370.12268892470334453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75700.049.8707241
GTATCAA130750.038.9774671
ATCAACG160350.031.1640553
TCAACGC160100.031.0948984
TATCAAC167200.030.1129472
CAACGCA167250.029.877655
AACGCAG175550.028.541966
TATAACG3600.026.1172562
ACGCAGA195600.025.2792367
CGCAGAG197100.024.8716358
GTACATG124150.023.8950861
GTGGTAT38500.023.0795361
GCAGAGT211400.022.6333859
TACATGG123700.022.5364272
TGGTATC38100.021.2228432
TACCTGG32950.021.1157442
ACATGGG125250.021.0184173
GAGTACT136850.020.43385512-13
AGAGTAC193700.019.92141710-11
CAGAGTA207100.019.6877510-11
Produced by FastQC (version 0.11.5)