FastQCFastQC Report
Thu 2 Jun 2016
DRR024863_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024863_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4187012
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111490.2662758071866047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100960.24112660770974625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62380.14898452643555835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75300.050.470941
GTATCAA130000.038.9670331
ATCAACG161600.031.0446823
TCAACGC162250.030.965694
CAACGCA168950.029.7376945
TATCAAC169600.029.6368182
AACGCAG176700.028.5128616
ACGCAGA201850.024.6796467
CGCAGAG203050.024.3717778
GTACATG125450.024.333191
GTGGTAT39850.023.0160771
TACATGG128850.022.3694742
GCAGAGT219000.022.1031889
ACATGGG127700.021.5391773
TGGTATC40600.020.8461652
TATAACG3650.020.6113682
AGAGTAC197000.020.1343710-11
GAGTACT143250.020.07791712-13
TACCTGG30750.019.4195122
ACAACGC6600.019.2346343
Produced by FastQC (version 0.11.5)