FastQCFastQC Report
Thu 2 Jun 2016
DRR024862_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024862_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6397958
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115690.18082331894019937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92280.1442335195073178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88750.051.3271941
GTATCAA167400.041.590271
ATCAACG200400.034.1287233
TCAACGC201450.033.950574
TATCAAC213150.032.1982652
CAACGCA216250.031.6265245
AACGCAG225350.030.389456
ACGCAGA248250.027.206637
CGCAGAG249100.026.9054018
GTACATG181000.026.8036061
TACATGG180100.024.9261172
GCAGAGT275150.023.8627539
GTGGTAT48400.023.6993561
ACATGGG186800.023.175953
TGGTATC46500.021.9367242
GTCGTAC3900.021.6970181
AGAGTAC248600.020.21963710-11
GAGTACT169950.020.1314512-13
CAGAGTA267200.019.55969210-11
AGTACTT178300.018.78011912-13
Produced by FastQC (version 0.11.5)