FastQCFastQC Report
Thu 2 Jun 2016
DRR024862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6397958
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130570.20408073951095018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104920.16398982300290188No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69100.10800320977411855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95700.052.6873781
GTATCAA175100.040.0780331
ATCAACG214300.032.2496723
TCAACGC218550.031.6121564
CAACGCA230150.030.0801035
TATCAAC231650.029.9777282
AACGCAG238800.029.0880176
GTGGTAT48500.025.0208281
ACGCAGA272300.025.0079337
CGCAGAG274250.024.641628
TGGTATC48500.024.6230872
GTACATG181650.023.847781
TACATGG178200.023.0597342
GCAGAGT301150.021.8319199
ACATGGG184850.021.4152343
AGAGTAC276050.019.92694510-11
GAGTACT191200.019.67571312-13
AGTACTT201550.018.87517712-13
CAGAGTA294100.018.74390210-11
GTACTTT212000.017.67876214-15
Produced by FastQC (version 0.11.5)