FastQCFastQC Report
Thu 2 Jun 2016
DRR024861_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024861_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792889
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86090.3082471233192583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67240.24075428704828586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47780.17107733246827928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54000.051.6234131
GTATCAA103850.042.369681
TACCTGG30500.040.225662
GTACCTG32750.038.325321
ACCTGGG35850.032.5175063
ATCAACG134400.032.4916043
TCAACGC135600.032.1688234
TATCAAC140250.031.2709642
CAACGCA142200.030.8735395
AACGCAG148850.029.65166
ACAACGC7150.026.9545763
ACGCAGA166850.026.1987347
CGCAGAG165550.026.1763948
GTACATG116700.025.57931
GTGGTAT26000.025.3128191
TACATGG112450.024.8725322
GTACCGG2850.024.741851
TGGTATC25150.024.4847552
GCAGAGT173800.024.4741049
GTACACG3250.023.1431481
Produced by FastQC (version 0.11.5)