FastQCFastQC Report
Thu 2 Jun 2016
DRR024861_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024861_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792889
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92770.33216500906409097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77170.27630886870190685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56020.20058083224933032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58500.054.3425451
GTATCAA113750.041.7973671
TACCTGG28250.039.6101532
TATAACG3300.038.4678462
GTACCTG32700.035.0906371
ACAACGC6350.032.5776063
ATCAACG147700.032.118183
TCAACGC149900.031.6052464
TATCAAC152300.031.1486532
CAACGCA155750.030.5992055
AACGCAG164000.029.0302166
ACCTGGG36500.028.9821013
GTACCGG3000.028.2163241
TGGTATC25600.028.0995562
GTGGTAT26900.027.6219251
GTACACG2650.026.6191731
ATAACGC4950.026.5945743
TACAACG8250.026.2151222
TAACGCA4700.026.0001434
ACGCAGA183500.025.636087
Produced by FastQC (version 0.11.5)