FastQCFastQC Report
Thu 2 Jun 2016
DRR024860_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024860_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4005227
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79760.19913977410019457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66630.16635761219027037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60500.052.6831741
GTATCAA108250.040.3880271
ATCAACG131450.032.5404973
TCAACGC132950.032.2083134
TATCAAC140800.030.7153632
CAACGCA139650.030.6290115
AACGCAG145350.029.5230456
ACGCAGA164850.025.6313067
CGCAGAG165100.025.5633878
GTACATG112350.025.273271
TACATGG111900.023.5669442
GCAGAGT176900.023.3799729
GTACGAC2853.6379788E-1223.090143
GTGGTAT30350.022.9245411
ACATGGG112750.022.7207833
TGGTATC30750.021.8605672
AGAGTAC164000.020.08923110-11
CAGAGTA174500.019.89043210-11
GAGTACT116900.019.61954912-13
CATGGGG72100.018.7104644
Produced by FastQC (version 0.11.5)