FastQCFastQC Report
Thu 2 Jun 2016
DRR024860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4005227
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86250.21534359975102535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76370.1906758343534586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47360.11824548271546158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60900.053.6051331
GTATCAA111250.040.041851
ATCAACG137500.031.8322283
TCAACGC139050.031.4007534
CAACGCA144700.030.3370745
TATCAAC148200.029.6302282
AACGCAG150500.029.1047526
GTACATG112200.025.5322231
ACGCAGA175100.024.7464547
ACCGCGT1901.3360477E-824.7351558
CGCAGAG176050.024.3993578
TACATGG112050.023.6228792
ACATGGG111700.022.6858793
GCAGAGT190000.022.1379629
GTGGTAT29700.021.5400471
TGGTATC29850.021.1055552
AGAGTAC175100.020.22391910-11
GAGTACT126250.019.50599712-13
CAGAGTA186350.019.16692410-11
AGTACTT130850.018.7843512-13
Produced by FastQC (version 0.11.5)