FastQCFastQC Report
Thu 2 Jun 2016
DRR024859_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024859_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4221146
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83360.19748191604839066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71700.1698590856606239No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.10051772670265373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65300.051.328121
GTATCAA120400.041.776911
ATCAACG147350.033.3360633
TCAACGC148750.033.0211374
TATCAAC155250.031.9137442
CAACGCA157150.031.2258115
AACGCAG164050.029.9969736
ACGCAGA183800.026.569117
CGCAGAG183650.026.3851348
GTACATG137900.026.1803821
TACATGG140250.024.7691042
GCAGAGT197250.023.8034579
GTGGTAT32650.023.0364651
ACATGGG147850.022.7953363
TGGTATC32700.022.2819252
GTACCGG2701.1423253E-920.8927941
AGAGTAC183650.020.6909610-11
GAGTACT121700.019.98485812-13
CAGAGTA194900.019.78601310-11
CATGGGG79900.019.5898084
Produced by FastQC (version 0.11.5)