FastQCFastQC Report
Thu 2 Jun 2016
DRR024859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4221146
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99330.2353152437750317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85660.20293067332899645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54910.13008315751220165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73000.052.0012661
GTATCAA135100.040.6678121
ATCAACG163650.033.0663153
TCAACGC166450.032.529074
TATCAAC172200.031.5883352
CAACGCA172650.031.306485
AACGCAG181550.029.7962426
ACGCAGA206400.025.9808437
CGCAGAG206500.025.8083348
GTACATG141000.025.5881881
TACATGG144800.023.9615172
GCAGAGT222800.023.3928629
GTGGTAT34650.022.6713051
ACATGGG149350.022.287153
TGGTATC35500.021.8515432
TATAACG4450.021.1298522
AGAGTAC204800.020.58398610-11
GAGTACT142250.020.0541112-13
TACCTGG33200.019.8251482
CAGAGTA218900.019.73043810-11
Produced by FastQC (version 0.11.5)