FastQCFastQC Report
Thu 2 Jun 2016
DRR024858_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024858_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2939612
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128230.4362140309673522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104690.35613543556088356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69090.23503101769893442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73500.055.390681
GTATCAA132650.041.5361751
TATAACG3500.033.577522
ATCAACG169500.032.168843
TCAACGC171650.031.793294
TATCAAC171800.031.7129692
GTGGTAT30900.030.7325821
CAACGCA181350.030.1699875
AACGCAG186300.029.5429696
TGGTATC32500.028.4943962
ACGCAGA207400.026.219237
TACCTGG25150.026.1677652
CGCAGAG210550.025.6483928
GTACATG157350.024.9175571
TACATGG157350.024.1988582
GCAGAGT216950.024.1764499
TAGCGCG1602.0385942E-623.502664
TAACGCA4300.022.9560874
ACATGGG160000.022.6506923
GTACCTG30800.022.1321411
Produced by FastQC (version 0.11.5)