FastQCFastQC Report
Thu 2 Jun 2016
DRR024858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2939612
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146710.49907947035186956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124400.4231851006187211No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87480.29759029422930644No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34690.11800877122559031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77400.052.93471
GTATCAA141550.042.7692761
ATCAACG186600.032.0771033
TATCAAC188850.031.7945232
TCAACGC188000.031.7746964
TATAACG2600.030.743552
CAACGCA195100.030.6906345
AACGCAG200700.029.9035286
GTGGTAT33250.028.5774151
ACGCAGA225000.026.4432817
CGCAGAG227500.026.0489648
GTACATG153950.025.1773911
TGGTATC37850.024.8452032
GCAGAGT240650.024.1959239
TACATGG154550.024.0345782
TACCTGG26650.023.289232
ACATGGG158900.022.666443
AGTACTT152400.021.431712-13
AGAGTAC226150.021.21709410-11
GAGTACT153400.021.2000812-13
Produced by FastQC (version 0.11.5)