FastQCFastQC Report
Thu 2 Jun 2016
DRR024857_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024857_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4439328
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105830.23839193679764145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89840.20237297176509597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54070.12179771352781321No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87800.049.045941
GTATCAA157800.039.7719041
ATCAACG192950.032.0382233
TCAACGC195200.031.6437764
TATCAAC202100.030.7977452
CAACGCA206100.030.0151755
AACGCAG214300.028.841816
GTACATG171400.027.043831
TACATGG172000.025.664592
ACGCAGA239150.025.3335827
CGCAGAG240250.025.0605168
ACATGGG178100.023.9403673
GCAGAGT256950.022.9010319
GAGTACT146150.021.03058612-13
GTGGTAT44950.019.8712881
AGAGTAC230850.019.67756710-11
CATGGGG101000.019.5013834
GTACTTT158650.019.41769814-15
CAGAGTA254750.019.1967310-11
AGTACTT154700.019.09358412-13
Produced by FastQC (version 0.11.5)