FastQCFastQC Report
Thu 2 Jun 2016
DRR024857_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024857_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4439328
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110960.24994773983810162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96560.2175103979701432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60350.13594399873133953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84500.051.3790051
GTATCAA150200.038.738291
ATCAACG192000.030.0709343
TCAACGC193300.029.6884024
TATCAAC199950.029.0637592
CAACGCA201150.028.5998945
AACGCAG209650.027.5063616
GTACATG174600.025.2696511
TACATGG171050.024.2987462
ACGCAGA238300.023.9421637
CGCAGAG238950.023.7983658
GTGGTAT41350.022.4094581
ACATGGG180600.022.0763823
GCAGAGT254750.021.80589
TACCTGG34850.021.0463352
TGGTATC42500.021.0193542
AGAGTAC231450.020.06173710-11
GAGTACT150650.019.88748212-13
GTACCTG40150.019.3303221
CATGGGG99650.019.0077514
Produced by FastQC (version 0.11.5)