FastQCFastQC Report
Thu 2 Jun 2016
DRR024856_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024856_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3056738
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84040.2749336056933895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69310.22674498108768235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41160.13465334614873764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57050.047.9589651
GTATCAA98300.039.0724831
ATCAACG118200.032.093413
TCAACGC118400.032.038154
CAACGCA123450.030.8032075
TATCAAC123500.030.75622
AACGCAG130350.029.4231536
TATAACG3550.027.8086052
ATAACGC3550.027.8067863
TACCTGG26300.027.1691512
GTACCTG28750.026.4898591
ACGCAGA144900.026.0469727
CGCAGAG145850.025.8115988
GTACATG85850.024.422891
GCAGAGT155950.023.6272079
TACATGG84100.023.532842
ACATGGG85000.022.286713
GTGGTAT29900.021.6975351
GAGTACT106750.021.37397412-13
TGGTATC30850.020.5715432
Produced by FastQC (version 0.11.5)