FastQCFastQC Report
Thu 2 Jun 2016
DRR024856_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024856_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3056738
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98570.32246793804375773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85380.2793173638041599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52160.170639420192375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57900.047.1142581
GTATCAA100850.040.2007641
ATCAACG126250.031.7650813
TCAACGC127650.031.407964
TATCAAC131300.030.6870772
CAACGCA132550.030.3532755
AACGCAG138700.029.0408156
TACCTGG26050.026.169762
GTACATG88750.025.2785971
ACGCAGA156750.025.2744587
CGCAGAG157050.024.9867828
GTACCTG29450.024.9139291
TACATGG86750.023.954782
GCAGAGT166200.023.1304519
ACATGGG89400.022.1925543
ACAACGC4650.021.2323823
GTGGTAT29150.021.1366251
AGAGTAC151050.020.81455810-11
TGGTATC29450.020.1152152
GAGTACT114100.020.07940112-13
Produced by FastQC (version 0.11.5)