FastQCFastQC Report
Thu 2 Jun 2016
DRR024855_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024855_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3182515
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90230.28351790957780243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74270.23336889221260543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46290.14545100337311842No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56250.048.2203141
GTATCAA105900.041.326681
ATCAACG130400.033.126363
TCAACGC132850.032.7272264
TACCTGG29650.031.8655052
TATCAAC136250.031.7395522
CAACGCA138500.031.4600165
AACGCAG143850.030.3865666
GTACCTG33850.029.1633471
GTGGTAT30000.027.5783441
ACGCAGA159950.026.9753427
CGCAGAG160750.026.5779488
TGGTATC30500.025.4292532
ACAACGC6600.024.926423
TACAACG6500.024.5875362
TAACGCA3850.024.4173364
GCAGAGT171850.024.2321939
GTACATG92200.024.2180671
TATAACG3200.023.5027922
TACATGG91150.022.9999892
Produced by FastQC (version 0.11.5)