FastQCFastQC Report
Thu 2 Jun 2016
DRR024855_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024855_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3182515
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102040.3206269255604451No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89140.28009294535925205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58740.1845710075207815No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61150.051.844981
GTATCAA112850.039.555591
ACAACGC6550.032.2995153
ATCAACG138800.031.8731443
TCAACGC140600.031.4891324
TATCAAC145750.030.5473142
CAACGCA146750.030.2975945
AACGCAG153300.029.033746
TACAACG8000.027.6210042
TACCTGG28900.026.8421752
GTACCGG3250.026.051481
GTGGTAT31250.025.588341
ACGCAGA173850.025.384327
CGCAGAG174850.025.1316288
GTACATG92700.023.4933281
TACATGG90600.023.0402072
TGGTATC31900.022.9914052
GCAGAGT185750.022.8219289
GTACCTG35600.022.4616761
ACATGGG91900.021.434993
Produced by FastQC (version 0.11.5)