FastQCFastQC Report
Thu 2 Jun 2016
DRR024854_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024854_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2352698
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86150.3661753442218253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70750.3007185792651671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43870.18646677134081807No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44850.052.197691
TACCTGG26650.047.2689322
GTATCAA87000.042.7947271
TATAACG2350.040.0037162
ACCTGGG31150.039.3840753
GTACCTG33100.038.9140971
GTACCGG3050.038.532271
ACAACGC5450.036.223553
ATCAACG110300.033.1544883
TCAACGC111250.032.786874
GTGGTAT20650.032.55371
CAACGCA115350.031.6615685
TATCAAC117650.031.1231672
AACGCAG120950.030.3504356
TGGTATC21750.029.6073472
GTACAAC7750.028.5089051
GTACCCG2550.027.6525671
CGCAGAG132950.027.2209768
ACGCAGA133150.027.2153857
GTATAAC5900.027.0901411
Produced by FastQC (version 0.11.5)