FastQCFastQC Report
Thu 2 Jun 2016
DRR024854_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024854_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2352698
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96000.40804217115838926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80710.34305295452284995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55210.2346667528089028No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45950.055.7788351
TATAACG2600.054.2460172
TACCTGG24000.049.559762
GTATCAA91050.042.0955431
GTACCGG2800.041.9894751
GTACCTG29050.041.7668761
ACCTGGG30500.037.4556353
TAACGCA4250.036.4911884
ACAACGC6350.034.056093
ATCAACG115900.032.6935653
TCAACGC116500.032.5141264
CAACGCA121950.031.4849665
TATCAAC122350.031.0859742
GTGGTAT20700.030.4433841
AACGCAG128550.029.9415866
TGGTATC20300.029.4122072
ATAACGC5300.029.2717553
GTACAAC8250.028.5019471
ACGCAGA143450.026.4384487
CGCAGAG144200.026.2357588
Produced by FastQC (version 0.11.5)