FastQCFastQC Report
Thu 2 Jun 2016
DRR024853_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024853_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049286
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73430.2408104716973088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61340.20116184575667875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38160.12514405011533847No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46450.051.0075571
GTATCAA88600.043.826421
TACCTGG35200.040.4632872
GTACCTG39100.037.7522241
TATAACG3000.037.6054882
ATCAACG110900.034.4168783
TCAACGC112050.034.1050384
ACAACGC6000.033.687143
ATAACGC3800.033.3985143
TATCAAC114800.033.2896962
CAACGCA117900.032.412275
AACGCAG123750.031.029956
GTCTCGC10100.029.323031
GTGGTAT22450.028.4781821
ACGCAGA134800.028.1715957
TGGTATC21550.027.9205442
CGCAGAG134950.027.861218
TAACGCA4750.027.7079414
TACAACG8050.026.2771242
ACCTGGG52550.026.208483
Produced by FastQC (version 0.11.5)