FastQCFastQC Report
Thu 2 Jun 2016
DRR024853_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024853_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049286
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85790.2813445508227172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72080.23638320577341715No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49840.1634480989976014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51150.054.9993441
TATAACG3600.045.712972
TACCTGG33550.040.2219622
ATAACGC3800.039.5943453
GTATCAA91650.039.5752681
GTACCTG36400.036.9629481
GTACCGG3300.035.6392061
ATCAACG113750.031.5797523
TCAACGC116400.030.8087794
TATCAAC118200.030.5106742
ACAACGC6350.030.3582613
CAACGCA122550.029.6845575
TAACGCA4750.029.6845554
GTCTCGC10050.029.0220171
AACGCAG129050.028.5522026
GTACATG74050.026.428361
ACCTGGG49600.025.973813
GTGGTAT22200.025.8528731
GTATAAC9400.025.5237371
ACGCAGA146350.025.079897
Produced by FastQC (version 0.11.5)