FastQCFastQC Report
Thu 2 Jun 2016
DRR024852_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024852_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194318
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202650.6344077202081947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160030.5009833084871325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107890.3377559779583623No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63730.19951050584193558No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47900.14995376164802626No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43200.13524013576606964No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32020.10024048951920253No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG35650.049.8492282
GGTATCA116700.048.911931
TATAACG4300.047.013892
TACAACG7650.044.8629262
ACCTGGG43900.039.1942523
GTATCAA204400.038.9212231
GTACCTG48200.037.3610231
TAACGCA6100.033.1383444
ATCAACG255800.030.9858063
TCAACGC258800.030.6256624
TATCAAC264200.030.0554352
ACAACGC11350.029.822393
ATAACGC6500.029.6535243
CAACGCA270350.029.3689655
AACGCAG281100.028.3245586
GTACAAC13250.027.6787011
GTACCGG3350.026.66711
GTACATG203650.026.3431821
TACATGG201750.025.889682
ACGCAGA312400.025.23017
Produced by FastQC (version 0.11.5)