FastQCFastQC Report
Thu 2 Jun 2016
DRR024851_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024851_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3690298
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141160.38251653389509466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118790.3218981231326034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73450.19903541665198854No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78500.055.2809451
GTATCAA144100.041.0123521
ATCAACG180200.032.5574383
TCAACGC181900.032.3298154
TATAACG3950.032.136912
TATCAAC188700.031.3184952
GTGGTAT32450.031.1506521
CAACGCA190200.030.9918865
TACCTGG34450.029.8876932
AACGCAG199350.029.7088496
TGGTATC33500.028.7704432
ACGCAGA223400.026.4042857
CGCAGAG226300.025.919838
GTACATG156850.024.3097061
GCAGAGT240100.023.588329
TACATGG155600.023.5679872
GTACCTG45250.023.48191
ATAACGC5450.023.2893663
ACCTGGG44750.022.7959213
TAACGCA5600.022.6649284
Produced by FastQC (version 0.11.5)