FastQCFastQC Report
Thu 2 Jun 2016
DRR024851_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024851_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3690298
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160050.4337048119149185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137920.3737367551346802No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94690.25659174408137225No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38250.10365016592155972No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78300.055.6915741
GTATCAA147700.042.0720671
GTGGTAT33300.032.6316681
TACCTGG34700.032.112822
ATCAACG191550.031.7863963
TCAACGC193850.031.4704994
TGGTATC33700.031.112472
TATCAAC197950.031.0203862
CAACGCA201750.030.2381975
AACGCAG211750.028.9203786
GTACATG151900.027.0041311
TACATGG150850.026.1191022
ACGCAGA238300.025.4401237
CGCAGAG240300.025.1892728
GTACCTG45450.025.1503431
ACATGGG157100.024.5110193
ACCTGGG44400.024.03793
GCAGAGT254900.023.2487059
TATAACG3850.021.9822082
ACAACGC8000.021.74533
Produced by FastQC (version 0.11.5)