FastQCFastQC Report
Thu 2 Jun 2016
DRR024850_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024850_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4443353
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117130.2636072353468203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99770.22453764083114708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57690.12983438407887016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75700.052.103431
GTATCAA141950.041.8281061
ATCAACG176600.033.2721183
TCAACGC179850.032.722414
TATCAAC185650.031.7528922
CAACGCA186800.031.5301155
AACGCAG195150.030.3709776
GTGGTAT35850.028.84921
TGGTATC34950.027.7077162
ACGCAGA218300.026.9130957
CGCAGAG219400.026.6484268
GTACATG135600.024.9616361
GCAGAGT234300.024.3720349
ACAACGC7150.024.3252243
TACATGG131900.024.05692
TACCTGG36550.023.2794062
ACATGGG132700.023.131483
GTACCTG40200.021.868341
AGAGTAC219200.020.80859810-11
CAGAGTA231350.019.95955510-11
Produced by FastQC (version 0.11.5)